Plasmid
lp

Part:BBa_K4427002:Design

Designed by: Quan Luo   Group: iGEM22_JLU_China   (2022-09-20)


LP，Our favourite part, which can output phenethyl alcohol


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 4820
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 4821
    Illegal PstI site found at 4835
    Illegal NotI site found at 7
    Illegal NotI site found at 4828
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 1165
    Illegal BamHI site found at 4743
    Illegal XhoI site found at 1743
    Illegal XhoI site found at 4752
    Illegal XhoI site found at 5852
    Illegal XhoI site found at 6744
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 4821
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 4821
    Illegal PstI site found at 4835
    Illegal NgoMIV site found at 1313
    Illegal AgeI site found at 1000
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References